Comparison of LGS/P-FILM with Other Protein Analysis Technologies

 

 

High throughput (more than 100 targets/day)

Detects 5 or more targets from multiple samples in parallel

Detects location of 5 or more targets in one tissue section

Uses ordinary lab equipment**

Can be used to measure nucleic acids as well as protein

Retains spatial relationships of targets in tissue section

Layered Membranes (20/20 GeneSystems)

Protein Microarrays (e.g., Prolinx)

N/A

SELDI/Mass Spectrometry (e.g., Ciphergen)

Tissue Microarrays (e.g., Zymed)

Western Blot (e.g., Bio-Rad)

N/A

IHC (e.g., USB) *

N/A

ELISA (e.g., Cell Sciences)

Flow Cytometry (e.g., Beckman Coulter) 

* Most Immunohistochemistry analyses are done in a ‘homebrew’ manner with reagents and instrumentation purchased from multiple suppliers.

**‘Ordinary lab equipment’ is defined as instruments that are found in the majority of research laboratories such as pipettors and gel boxes.