20/20 GeneSystems announces the recent successful use of its Layered Proteomics Technology in the analysis of a bovine mammary gland tissue sections. The company is particularly pleased as the specimen was embedded in plastic, a medium notorious for interference with protein analysis. Immmunoanalysis of the membranes was done by Dr. Steven Ellis of the United States Department of Agriculture. Here, Dr. Ellis describes his results:
'I was able to successfully detect the alpha-form of the bovine estrogen receptor [from bovine mammary gland tissue] on one of the membranes you sent. [20/20 GeneSystems] transferred the proteins out of a 2 micron glycol methacrylate histology section onto about 6 membranes. I stained the first one in the series with 1:1000 dilutions of primary and HRP-conjugated secondary antibodies. ECL was done with the Pierce SuperSignal-West kit. So, next time someone asks whether it'll work, you can tell them "Yes".'
'Also, I stained the slides (post-transfer) and saw essentially no change in the histologic staining with Azure II (so photomicrography would still be an option for peole interested in doing so). We will be in contact with you in the future when we have need of replicate membrane blots.'
'It would certainly appear that your system works as advertised.'