Comparison of Version 2

Improved membrane performance!

	Better consistency membrane to membrane: the amount of protein bound to the blots is proportional to their distance from the sample.
	Less variability (15%) in amount of protein bound blot to blot.
	Exceptionally low background.

View other examples of new membrane performance

Comparison of Version 2.0 P-FILM to PVDF and original P-FILM (Version 1.0)

Comparison of Version 2.0 P-FILM to Version 1.0 and PVDF. 5-membrane stacks of P-FILM (‘Version I.0’ and the new improved ‘Version 2.0’) were run side by side with PVDF. Two cell lysates (Jurkat and HaCaT) were separated on a 4-20% gradient gel (Bio-Rad) and transferred to PVDF, Version I.0 or Version 2.0 P-FILM membranes using a mini-gel tank transfer device (Bio-Rad). After transfer, the P-FILM stacks were cut in half lengthwise. Membranes were first stained to visualize total protein with either Ponceau S (PVDF) or Chemicon’s FastStain (P-FILM). The Version 2.0 membranes showed substantially better signal intensity than Version 1.0 P-FILM. Variability was 10-15% membrane to membrane with the Version 2.0 membranes and 25-30% through the Version 1.0 stack. Variability across the membranes (intramembrane variability) was less than 5% for both Version 1.0 and Version 2.0 (data not shown). Next, membranes were probed with antibodies to three proteins, one of high abundance (GAPDH), two of low to moderate abundance (p53 and p-AKT) in the two cell lines tested. All blots were incubated in the same antibody solution; protein presence was visualized with ECL-Plus™ enhanced chemiluminescence reagent (Amersham-Pharmacia). The Version 2.0 P-FILM showed better sensitivity and more consistent signal blot to blot. The high and moderate to low abundance proteins were detected on all Version 2.0 blots.

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